Freezing-modulated-crosslinking: A crosslinking approach for 3D cryoprinting

نویسندگان

چکیده

3D Bioprinting is a popular method of fabricating scaffolds for tissue engineering. While soft bioinks such as alginate are useful reproducing the softest tissues human body, it remains difficult to print objects out that cannot support their own weight. cryoprinting promising printing and creating highly porous However, crosslinking frozen before they thaw collapse challenge. Here we investigate process which name “freezing-modulated-crosslinking” produced by cryoprinting. During freezing-modulated-crosslinking, thawed in crosslinker bath at controlled melting rate, so occurs layer object can maintain its printed shape. First, examine with mathematical model determine important thermal parameters. Second, validate our results experimentally variety multi-layer objects. By systematically examining this approach, expand options cryoprinted be seeded cells use cell culture or regeneration.

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ژورنال

عنوان ژورنال: Bioprinting

سال: 2022

ISSN: ['2405-8866']

DOI: https://doi.org/10.1016/j.bprint.2022.e00225